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i scei expression vector pcba scei  (Addgene inc)


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    Addgene inc i scei expression vector pcba scei
    I Scei Expression Vector Pcba Scei, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 293 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/i+scei+expression+vector+pcba+scei/pCBASceI+(Plasmid+%2326477)/pmc06795472-236-20-24
    Average 96 stars, based on 293 article reviews
    i scei expression vector pcba scei - by Bioz Stars, 2026-09
    96/100 stars

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    Transfection:

    Article Title: Differential Requirements for the RAD51 Paralogs in Genome Repair and Maintenance in Human Cells
    Article Snippet: Post-Cre MCF10A cells were infected with I-Sce-I expressing lentivirus, cells were washed 24 h later and HR was measured 48 h later by quantifying the percentage of GFP+ cells by FACS (Becton Dickinson FACScan). .. U2OS and HEK293 cells were seeded into 10 cm plates 24 h prior to transfection with 2.5 μg of the I-SceI expression vector pCBA-SceI (Addgene plasmid # 26477, ) or an empty vector in combination with 0.5 μg of pcDNA-RFP using polyethylenimine (PEI) at a ratio of 2:1 of PEI:total DNA. .. Cells were collected 72 h post-transfection and multicolor FACS analysis was performed with a LSRII apparatus (Becton Dickinson).

    Article Title: Differential Requirements for the RAD51 Paralogs in Genome Repair and Maintenance in Human Cells
    Article Snippet: Post-Cre MCF10A cells were infected with I-Sce-I expressing lentivirus, cells were washed 24 h later and HR was measured 48 h later by quantifying the percentage of GFP+ cells by FACS (Becton Dickinson FACScan). .. U2OS and HEK293 cells were seeded into 10 cm plates 24 h prior to transfection with 2.5 μg of the I-SceI expression vector pCBA-SceI (Addgene plasmid # 26477, [ ] or an empty vector in combination with 0.5 μg of pcDNA-RFP using polyethylenimine (PEI) at a ratio of 2:1 of PEI:total DNA. .. Cells were collected 72 h post-transfection and multicolor FACS analysis was performed with a LSRII apparatus (Becton Dickinson).

    Expressing:

    Article Title: Differential Requirements for the RAD51 Paralogs in Genome Repair and Maintenance in Human Cells
    Article Snippet: Post-Cre MCF10A cells were infected with I-Sce-I expressing lentivirus, cells were washed 24 h later and HR was measured 48 h later by quantifying the percentage of GFP+ cells by FACS (Becton Dickinson FACScan). .. U2OS and HEK293 cells were seeded into 10 cm plates 24 h prior to transfection with 2.5 μg of the I-SceI expression vector pCBA-SceI (Addgene plasmid # 26477, ) or an empty vector in combination with 0.5 μg of pcDNA-RFP using polyethylenimine (PEI) at a ratio of 2:1 of PEI:total DNA. .. Cells were collected 72 h post-transfection and multicolor FACS analysis was performed with a LSRII apparatus (Becton Dickinson).

    Article Title: Differential Requirements for the RAD51 Paralogs in Genome Repair and Maintenance in Human Cells
    Article Snippet: Post-Cre MCF10A cells were infected with I-Sce-I expressing lentivirus, cells were washed 24 h later and HR was measured 48 h later by quantifying the percentage of GFP+ cells by FACS (Becton Dickinson FACScan). .. U2OS and HEK293 cells were seeded into 10 cm plates 24 h prior to transfection with 2.5 μg of the I-SceI expression vector pCBA-SceI (Addgene plasmid # 26477, [ ] or an empty vector in combination with 0.5 μg of pcDNA-RFP using polyethylenimine (PEI) at a ratio of 2:1 of PEI:total DNA. .. Cells were collected 72 h post-transfection and multicolor FACS analysis was performed with a LSRII apparatus (Becton Dickinson).

    Plasmid Preparation:

    Article Title: Differential Requirements for the RAD51 Paralogs in Genome Repair and Maintenance in Human Cells
    Article Snippet: Post-Cre MCF10A cells were infected with I-Sce-I expressing lentivirus, cells were washed 24 h later and HR was measured 48 h later by quantifying the percentage of GFP+ cells by FACS (Becton Dickinson FACScan). .. U2OS and HEK293 cells were seeded into 10 cm plates 24 h prior to transfection with 2.5 μg of the I-SceI expression vector pCBA-SceI (Addgene plasmid # 26477, ) or an empty vector in combination with 0.5 μg of pcDNA-RFP using polyethylenimine (PEI) at a ratio of 2:1 of PEI:total DNA. .. Cells were collected 72 h post-transfection and multicolor FACS analysis was performed with a LSRII apparatus (Becton Dickinson).

    Article Title: Differential Requirements for the RAD51 Paralogs in Genome Repair and Maintenance in Human Cells
    Article Snippet: Post-Cre MCF10A cells were infected with I-Sce-I expressing lentivirus, cells were washed 24 h later and HR was measured 48 h later by quantifying the percentage of GFP+ cells by FACS (Becton Dickinson FACScan). .. U2OS and HEK293 cells were seeded into 10 cm plates 24 h prior to transfection with 2.5 μg of the I-SceI expression vector pCBA-SceI (Addgene plasmid # 26477, [ ] or an empty vector in combination with 0.5 μg of pcDNA-RFP using polyethylenimine (PEI) at a ratio of 2:1 of PEI:total DNA. .. Cells were collected 72 h post-transfection and multicolor FACS analysis was performed with a LSRII apparatus (Becton Dickinson).



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    Addgene inc i scei expression vector pcba scei
    I Scei Expression Vector Pcba Scei, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/i+scei+expression+vector+pcba+scei/pCBASceI+(Plasmid+%2326477)/pmc06795472-236-20-24
    Average 96 stars, based on 1 article reviews
    i scei expression vector pcba scei - by Bioz Stars, 2026-09
    96/100 stars
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